Effect of Dolichos Biflorus on Blood Sugar and Lipids in Diabetic Rats.

Diabetes mellitus is a chronic disease characterized by hyperglycemia and dyslipidemia, which leads to severe morbid complications. In view of the effectiveness of conventional herbal adjutants the present study was taken to assess the anti-diabetic and anti-lipidemic effect of Dolichos biflorus. 32 healthy albino rats of either sex were randomly divided into 4 equal groups (I–IV) each having 8 rats. Group I, control rats received only vehicular fluid while Group II received only Dolichos biflorus for the experimental period of 30 days. Diabetes was induced in Group II and Group IV rats by single intravenous dose of STZ and confirmed on 3rd day with fasting blood sugar (FBS) more than 250 mg/dl. Group III diabetic rats, received vehicular fluid while Group IV diabetic rats received Dolichos biflorus in single dose of 300 mg/kg body weight/day intra-gastrically up to the experimental period of 30 days. FBS levels were assessed on 1st, 8th, 15th, 22nd and 30th day. On last day overnight fasted rats were sacrificed for drawing the sample for lipid profile from beating heart and harvested for pancreatic tissue histology. Data obtained showed that FBS levels were decreased significantly when Dolichos biflorus was given to diabetic rats from mean±SD values 362±63.36 to 118±38.55 with p value < 001.S cholesterol and S. triglyceride levels were also decreased significantly with P value < 001. It was concluded that Dolichos biflorus has anti-diabetic and anti-lipidemic effect at daily oral dose and can be used as an adjuvant for management of diabetes mellitus and its associated complications.

Effects of curcumin on the gastric emptying of albino rats.

Curcumin (diferuloylmethane), a polyphenol, is an active principle of the perennial herb Curcuma longa commonly known as turmeric. Turmeric (CURCUMA LONGA L.) is a medicinal plant extensively used in Ayurveda, Unani, and Siddha medicine as a home remedy for various diseases including biliary diseases, cough, hepatic diseases, wound healing. However studies on the effect of curcumin on the gastric emptying are nearly nonexistent. It is hypothesized that curcumin may have an effect on gastric emptying. For this reason the present study was aimed to study the effect of curcumin on gastric emptying. Rats were divided into 5 groups (Group I – Group V), based on the time interval between administration of curcumin/vehicular fluid to administration of barium sulphate (Group I – 1 hr, Group II – 8 hrs, Group III – 16 hrs, Group IV – 24 hrs, Group V – 48 hrs). Each group was further divided into two subgroups, Group A (control) and Group B (experimental), containing 6 rats each. Rats in experimental group were administered curcumin intragastrically, in the dose of 1 gm/kg body weight, suspended in normal saline (0.9% NaCl). The controls were given vehicular fluid intragastrically, in volume equal to the experimental animals. It was observed that there was a decrease in the gastric emptying in all the experimental groups.

Adiposity: Determinant of peak expiratory flow rate in young Indian adults male.

Background. Although several factors such as respiratory muscle strength, lung compliance, resistance to airflow, and even obesity affect the lung functions, the nature of relationship with markers of adiposity is not clear. We hypothesised that central pattern of fat distribution is a significant predictor of decreased peak expiratory flow rate (PEFR). The present study was designed with the aim to examine the effects of adiposity on PEFR in males. Methods. One hundred young healthy male volunteers were analysed in the study. They were classified into non-obese, and obese groups based on body mass index (BMI) (obese ≥30Kg/m2 and non-obese <30Kg/m2). The PEFR was measured by using Wright’s peak flow meter. Data was analysed using unpaired ‘t’ test for statistical significance of differences between the non-obese and the obese, stratified into age groups of 20 to 30 years and 30 to 40 years. A partial correlation adjusted to age, height and BMI followed by regression analysis was conducted using adiposity markers as a predictor of PEFR. Results. The model adjusted to age, height, weight and BMI revealed waist hip ratio (WHR) as the only parameter which shows significant variance in PEFR with a Pearson’s r=-0.59, F (1, 100)=12.23, p=0.04. The resulting linear regression equation is y=-388.72xWHR+850.68. Conclusions. Our findings suggest that obesity itself and especially the pattern of body fat distribution have independent effects on PEFR. These results suggest that abdominal adiposity, measured as WHR, is a better predictor of expiratory flow than weight or BMI.

Effects of curcumin on the intestinal motility of albino rats.

Curcumin has been used in traditional medicine as a household remedy for various diseases including biliary diseases, cough, hepatic diseases, wound healing. For past few decades, extensive work has been done on biological activities of curcumin. This study was carried out to provide scientific basis for the use of curcumin in gastrointestinal disorders. Animals were divided into 5 groups (Group I - Group V), based on the time interval between administration of curcumin/vehicular fluid to administration of barium sulphate (Group I – 1 hr, Group II – 8 hrs, Group III – 16 hrs, Group IV – 24 hrs, Group V – 48 hrs). Each group was further divided into two sub-groups, Group A (control) and Group B (experimental), containing 6 rats each. Rats in Group A were given vehicular fluid (0.9% NaCl) while the rats in Group B were administered curcumin intragastrically by the naso-gastric tube reaching up to the lower 1/3rd of esophagus, in the dose of 1 gm/kg body weight, suspended in normal saline. After the intra-gastric administration of single dose of curcumin, there was decrease in length of small intestine traversed by BaSO4 in all the experimental groups as compared to control groups. These data suggests that curcumin decreases intestinal motility in albino rats, and this may partly explain the traditional use of curcumin in different disorders like diarrhea, abdominal cramps and irritable bowel syndrome.